Multi-Well MEA Biochips

We now offer the possibility to carry out multiple cell culture experiments in parallel on the same biochip, through our new Multi-Well MEA biochip, whose dimensions and configuration conform closely to that of the standard 96-well microplate format. The first generation of the Multi-Well Biochip comprises four wells per chip, where each well consists of 15 recording/stimulation electrodes, 1 large embedded ground electrode and 2 supplementary larger stimulation electrodes.

We welcome any enquiries and feedback on this product, as well as questions on customized biochip layouts.

The picture on the right displays the layout of the current 4-well MEA Biochip. Black: the electrodes, dark grey: Plastic insert defining the wells, light grey: electrode wires.

Blown-up version of a single well, displaying the large ground electrode, supplementary stimulation, and standard recording/stimulating electrodes.

Picture of a Multi-Well MEA Biochip inside a MEA1060 amplifier from Multi Channel Systems MCS, Reutlingen, Germany. Note the use of an MEA1060-Y1 accessory to contact the embedded ground and supplementary stimulation electrodes.

Typical recordings of spontaneous electrical activity from dissociated hippocampal neurons (from P1 rat, 31 days in vitro) on a 4-Well MEA Biochip, where each quadrant represents data from each well.

Electrical recording from one electrode located in the left-bottom quadrant of the data displayed in the picture above.

MEA60 4Wells

Number of wells: 4
Electrodes per well: 15 recording electrodes, 2 external stimulation electrodes and 1 large GND electrode

Substrate dimension: 21 mm x 21 mm x 0.7 mm
Substrate material: float glass
Insulation material: SU-8 epoxy, thickness 5 µm
Electrode material: platinum
Electrode geometry: planar
Electrode dimensions: 40 µm circular electrodes
Electrode spacing: 200 µm (centre to centre)
Electrode impedance at 1 kHz: 500-700 kΩ, GND electrode 450 Ω, stimulation electrodes 20-30 kΩ
Noise level: 20-25 µV

Applications: Dissociated cell cultures (brain tissue, spinal cord, retina, heart muscle cells, etc.)
Re-use: Several times depending on type and duration of cultures

Required accessories: MEA60 Spa-Y2 and MEA1060-Y1 or MEA1060-Y2 depending on MEA1060 amplifier from Multi Channel Systems MCS GmbH.